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Vi and viv domains responsible for lcdd and amyloidosis and v domains from non-pathogenic lc were modeled using the promod automatic molecular modeling server which includes the following model building steps peitsch and jongeneel, 1993; peitsch, 1995, 1996 ; : i ; building an average framework blundell et al., 1987 ; from templates available in the pdb eck and sprang, 1989; jones et al., 1989 ; ; ii ; fitting the backbone to be studied on to this framework, using a primary sequence alignment optimized for 3d similarity; iii ; building loop regions by structural homology searches in the pdb greer, 1990 ; ; iv ; rebuilding missing side chains using a library of allowed rotamers ponder and richards, 1987 ; ; v ; optimizing bond geometries and relief of unfavorable nonbonded contacts by energy minimization using the charmm program brooks et al., 1983 ; to relax major steric strains; vi ; analysis of the resulting model using the 3d profile matching procedure of luthy et al. 1992 ; . structures used as templates are listed in table ii, with corresponding resolutions, r values and percentages of identity with studied sequences. the procheck software laskowski, 1993; laskowski et al., 1993 ; was used to validate the models, by testing all basic geometric parameters and also proposing a secondary structure assignment according to kabsch and sander 1983 ; . a model of the non-pathogenic lc v domain len tlen ; , the structure of which has been determined by x-ray crystallography xlen ; huang, d.b., chang, c.h., ainsworth, c., johnson, g., solomon, a., stevens, f.j., schiffer, m., submitted ; , was obtained as described above. tlen was compared with its corresponding region of the x-ray structure. for cdr comparison, the direct r.m.s. value was calculated bajorath et al., 1995; bajorath and sheriff, 1996 ; . residues in these loops were defined according to chothia and lesk 1987 ; and chothia et al. 1989 ; . surface accessibilities to the solvent were calculated lee and richards, 1971 ; . the relative accessibility of each residue was obtained by dividing its corresponding absolute accessibility with that of the same residue in an extended conformation richmond and richards, 1978 ; . results and discussion sequence analysis detailed analysis revealed unusual residues at several positions figure 1 ; . unusual residues were defined as occurring in less than 1% of compared sequences at the corresponding position. twelve out of the 19 unusual residues in the whole set of lcdd sequences were found to be located in the cdrs. however, they were equally frequent in the frs and cdrs of proteins ise and sci and more frequent in frs of protein mcm. because the kabat databank contains many sequences of human lcs which are not secreted as free subunits, we also compared our data with a database including known monoclonal lcs from patients with plasma cell dyscrasias f evens, unpublished data ; . results are given in table iii. it is worth noting that sequences of al-amyloidosis lcs are largely overrepresented in this databank 51% of chains ; as compared with the normal incidence in pathology. interestingly, l27 was found in both ise and blu but in none of the v domain sequences of the kabat databank and.
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Overview a combo guard who can score in a number of ways. has the ball handling abilities to run the point. brings toughness and tenacity. has the potential to be solid defensively. canada exhibition fall 2004 ; averaged 9.0 points in four games on the ku 4-0 trip in british columbia, canada. scored 12 points in each of the first two games against university of british columbia and langara college. had five assists in three of the four games and was second on the ku team with 18 assists. high school rice high school 2004 ; . coached by maurice hicks. averaged 22 points and eight rebounds as a senior. new york newsday 2004 manhattan player of the year. new york daily news 2004 all-city team selection. 2004 chsaa all-city team honoree. as a junior, led his team with 18 points, six rebounds, four assists and four steals per game. helped his school to a state championship in 2002. rated the eighth-best shooting guard and 31st-best prospect overall by rivals . off the court led his summer basketball team to three tournament championships, being named mvp of each of those tournaments. joins brett ballard and brett olson as recent jayhawks to don the number three.
Fellow vr founder wore, but.four years. dammit, had they really gone that long without saying much, and not seeing each other? minerva smiled. "you like it? i had it custom built, and it's almost paid for." katze blinked. "uh, what?" "long story, " minerva said airily. "it's good to see you too, katze. it looks like college life agrees with yo- hey!" minerva grasped katze's left hand and examined her ring. "he finally proposed! cool! when did this happen?" "last week, on my birthday." a small part of katze's mind wondered exactly what minerva meant when she said "finally, " and exactly how much everybody knew about her and josh. the rest of her was surprised by the feel of minerva's hand. it actually felt real, not like a projection or something artificial. minerva sensed katze's surprise and looked up at the taller woman. "let me guess, you were expecting a hologram, right? or maybe a plain boring old robot?" minerva grinned. "dad never did like that star trek gag with the holograms, and i didn't want to be stuck in another bloody metal shell. this is pure 100% human genome, right down to the bone. except what's in here, " minerva tapped the side of her head, "that's nothing but vrdet original optical computer. i've got to maintain standards, you know." katze shook her head. "i. wow. color me impressed. i didn't know mal could do stuff like that." minerva laughed. "neither did he, at least until we got started." she guided katze towards the lone door waiting at the end of the lobby. "c'mon, we can catch up on old times later, the boss man awaits." they went through the only other door in the lobby, and stepped into a rather well apportioned office, with windows everywhere. katze blinked at the view. it wasn't her beloved san francisco, but it was rather picturesque at that, and she'd grown fond of colorado in her years of service to the cause. "goddamn, " she said. the chair rotated around so the figure in it could face her. mal always did have a sense of showmanship. "it is rather striking, no?" he said, and the voice was right but the general figure was wrong. "gah, " katze said, trying to find words. "i mean.i know that's you, but i'm still not used to it being you, even though i see you on the news all the time with that face." she dropped off, figuring she'd get used to it at some point in time. "hmm? oh.sorry, " mal said, and did something that rather surprised katze, even when she thought she could no longer be surprised by anything her old cohort was capable of. he changed back into the face she was familiar with right then and there in what appeared to be the space of an eyeblink. "is that better?" he asked. "showoff, " katze said, with a smile and a hint of admiration. "although i'm going to take a wild guess and figure that's how you managed to play lyran in marraketh." mal smirked. "that old gag? nah, that was just a mask and a cheap bathrobe, " he said. "i didn't get the face-changing thing set up until right before the stand down and online casino gambling.
Do you have a cordless drill capable of drilling masonry? sam would like to borrow a strong portable drill for one or two days in mid january. are there any bee keepers on the island? if so please get in touch with sam as he needs some advice so he can help the parrots without getting stung! if you have parrots near your house after 1800hrs can you let sam know. he is interested to find any new areas where they are roosting. contact sam by email at sam.williams shef.ac , or leave your phone number with the reporter 786-6125 786-6518 ; so they can call you when sam lands on the island. flotsam is continued on page 8.
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Elk's lodge #48 members: it's time to stop practicing your golf swing with your snow shovel spring is finally here! lets get our golf clubs out of hibernation and head to the highlands for a round of golf. there are many events planned for the 2008 golf season. please stop by or call the golf shop 453-1504 to sign up for the elk's golf league, thursday golf member men's league, the men's opening scramble, and the ladies golf association. thursday night men's league play begins on april 24th for the golf member men's league. many two-man teams have already signed up. please hurry before the league rosters are full. men's opening scramble saturday, april 26th kicks off the 2008 event schedule. a continental breakfast precedes the four-person scramble. please join us for a lunch buffet following golf to collect on your bets. elk's golf league this tuesday morning league is open to any member of the elks. pick your own partner or sign-up as a single and we will find you a partner. why not introduce a friend to the elks sign them up on your two-man team. the league will play at 9: 00 a.m. on tuesday mornings beginning may 6th. ladies golf association the ladies golf association begins play on may 7th. please contact the golf shop if you will be joining the ladies in 2008. there will be a wednesday league at 9: 30 a.m. and an evening league at 5: 00 p.m. don't forget about our membership packages. full memberships are available for those of you who play a lot of golf. limited memberships are a great value for those who play fewer rounds. and, corporate memberships are now available for those of you who use golf to entertain clients. we will treat your clients as well as you do. check the condition of your golf club's grips. if they are slippery or worn, bring them to the golf shop for replacement. we have many new styles of grips in stock at very reasonable prices. steve scheuermann pga golf professional and free casino.
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1. ratification of the minutes the vice chair requested a motion to approve the minutes of the october 16, 2007 members meeting. ms. patricia campos requested that the minutes be amended to clarify her statement regarding the atlantic city international airport expansion project, such that the minutes reflect her reference to an increase in business travel from outside the state instead of travel from northern new jersey, as stated in the minutes. a motion to ratify the minutes, as amended was made by mr. dalton and seconded by mr. cammarano. mr. amato and mr. walsh, who were not present for the previous meeting, abstained from the vote. resolution no. 07 was approved by a vote of 130. 2. treasurer's report the vice chair asked the members to review the treasurer's report. a motion to approve and file the report was made by mr. gant and seconded by ms. lusk. by a vote of 150, the report was received. 3. executive director's report executive director thomas carver welcomed mr. michael walsh of harrah's entertainment and mr. nicholas amato of resorts international as the new casino industry representatives to the authority. mr. carver also briefly reviewed two items in the agenda concerning the establishment of funds for the transportation master plan project. atlantic city project review ms. ali reynolds, chair of the atlantic city project review committee advised that the items on today's agenda were reviewed by committee and recommended for approval by the members. 1. resolution of the casino reinvestment development authority authorizing the establishment of the atlantic city transportation and green sustainable initiatives fund to receive deposits from the crda's share of certain tax rebates from atlantic city entertainment retail district projects the resolution was read by title. ms. susan thompson, chief operating officer, provided a description of the proposed action. the vice chair elicited discussion from the members and then opened the matter to the public for comment. mr. william crane, director of planning for the city of atlantic city expressed his support for the transportation master plan project and his concern.
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Aap 28 april 2006 a socceroos player has won over , 000 in damages from a european club at fifa's dispute resolution chamber. the player - who did not want to be named - was represented by the australian professional footballers association pfa ; which says his case serves as a warning to others looking to move abroad. the club had undertaken in writing to sign the player but at a late stage opted out of the deal when its circumstances changed, leaving the player without a club. the pfa says fifa ruled there was no enforceable contract between the club and the player, however it agreed the club's conduct was dishonest and prevented the player from looking elsewhere for a contract during the transfer window. "there remains a culture of subterfuge within european football from which players require protecting and about which they require education. this protection and education represents an important part of the pfas role, said pfa chief executive john didulica. "australian players hoping to move abroad need to be careful not to be drawn into a nod-nod, wink-wink mentality. "fortunately in this case, the pfa had the expertise to extract a result for the player however prevention is better than a cure. "as soon as young players enter the system, the game needs to arm them with knowledge. "each player needs to be educated that as soon as they decide to become a professional footballer, they become a small business in a billion-dollar industry. they will need to deal with contracts, agents, colleagues and clubs often all at once and in a foreign language." the player requested not to be named. since the inception of the dispute resolution chamber in 2002, the pfa has secured close to a million dollars in unpaid wages and damages for australian footballers playing overseas.
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